The Department of Medicine, Division of Gastroenterology is seeking a **Research Technologist** to independently collect, organize, and present data with the supervision of a principle faculty investigator, as well as contribute to the general maintenance of the laboratory, equipment, and supplies. **Specific Duties and Responsibilities** + Analyze DNA methylation data obtained via methylation-specific PCR as well as by methyl-deep sequencing. + Help develop algorithms and panels based on data. + Develop expertise in computer coding and programming, particularly using the programs R and Python. + Have familiarity with Barrett’s esophagus, esophageal adenocarcinoma, and esophageal squamous cell carcinoma diagnosis. + Perform gene expression assays and assist in molecular cloning experiments. + Perform standard protein assays to determine the effects of experimental manipulations on target protein levels. + Assist in small animal model husbandry and experiments. + Learn basic laboratory techniques such as pipetting, buffer and reagent preparation, pH determination, qPCR, RT-qPCR, western blotting, ELISA spectrophotometric absorbance determination and fluorescence detection. + Operate basic laboratory equipment such as centrifuge, pH meter, analytical balance, scintillation counter, light and/or fluorescence microscope, microplate reader, plate washer and biological safety cabinet. This person may also utilize sophisticated instrumentation, spectrophotometer, thermal cycler, flow cytometer, and laboratory automation solutions/robotics. Responsible for operation of lab computers for data entry. + Maintain accurate data records and laboratory notebook. Maintains and makes necessary adjustments to equipment, e.g. calibrating, alignment. Prepares solutions, reagents, and stains following standard laboratory formulas and procedures. Along with the laboratory supervisor, works to develop and institute standard operating procedures. **Minimum Qualifications** + Bachelor's Degree in Biology, Chemistry or related field. + Additional education may substitute for required experience and additional related experience may substitute for required education beyond HS Diploma/Graduation Equivalent, to the extent permitted by the JHU equivalency formula. **Preferred Qualifications** + Related post-undergraduate work experience Classified Title: Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Salary Range: $15.70 - $26.25 HRLY ($43,680 targeted; Commensurate w/exp.) Employee group: Full Time Schedule: M-F Varies FLSA Status: Non-Exempt Location: Hybrid/School of Medicine Campus Department name: SOM DOM Gastroenterology Personnel area: School of Medicine The listed salary range represents the minimum and maximum Johns Hopkins University offers for this position, based on a good faith estimate at the time of posting. Actual compensation will vary depending on factors such as location, skills, experience, market conditions, education, and internal equity. Not all candidates will qualify for the highest salary in the range. Johns Hopkins provides a comprehensive benefits package supporting health, career, and retirement. Learn more: https://hr.jhu.edu/benefits-worklife/. Equal Opportunity Employer All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran. EEO is the Law https://www.eeoc.gov/sites/default/files/2023-06/22-088_EEOC_KnowYourRights6.12ScreenRdr.pdf