The Division of Pulmonary is seeking a **_Research Technologist to_** provide operational support in the Sidhaye Lab. The technologist will oversee the maintenance of the mouse colonies. In addition, will utilize a foundation in basic laboratory techniques to work with molecular biology and primary cell culture requiring a high level of rigor and reproducibility. Assist with the planning of experiments and interpreting results. Perform data collection, prepare reports and interface with sponsors for laboratory projects. **Specific Duties & Responsibilities** + Perform mouse breeding and husbandry. + Perform general molecular biology techniques such as cloning, qPCR, and Western blotting. + Conduct experiments involving propagation of tissue culture, plasmid constructions, gel electrophoresis, immunoblotting, immunohistochemical staining of cells and tissue, and RT-qPCR. (Research involves animal and human tissue). + Follow established procedures or protocols and utilizes aseptic technique to perform laboratory tests and/or experiments which may include basic tissue culture, basic molecular biology, and specialized histology techniques. + Operate basic laboratory equipment such as centrifuge, pH meter, analytical balance, incubator, spectrophotometer, light microscope, and/or utilizes laminar flow hood. + Operate lab computers for data entry. + Utilize in vivo models in experiments. Following protocols, monitor animals and maintain health records in accordance with USDA and AALAC guidelines. + Sacrifice and dissect animal remains as required. + Prepare tissue for study utilizing biochemical and histochemical techniques. + Maintain accurate data records. + Maintain and make minor adjustments to equipment, e.g., calibrating, alignment. + Comply with biohazard/radiation safety standards through proper handling of potentially hazardous chemical and biological agents and/or radiation sources in the workplace. + Complete annual university biohazard/universal precaution/radiation safety training, as appropriate. + Use sterile techniques to avoid contaminating lab experiments. + Use universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens. + Prepare solutions, reagents, and stains following standard laboratory formulas and procedures. + Monitor inventory levels, order materials and supplies, count orders upon receipt. + Perform multiple tasks efficiently and correctly, often operating more than one instrument at a time. + Provide training to other individuals who may be assigned to the laboratory on a regular or temporary basis. **Minimum Qualifications** + Bachelor's Degree in biology, chemistry or related field + Experience in basic laboratory procedures. + Additional education may substitute for required experience and additional related experience may substitute for required education beyond HS Diploma/Graduation Equivalent, to the extent permitted by the JHU equivalency formula. **Preferred Qualifications** + Experience working with in vivo models + Some related post-undergraduate work experience. + Experience in a comparable laboratory, or a significant independent research project, not related to normal course requirements. Classified Title: Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Salary Range: $15.70 - $26.25 HRLY (Commensurate w/exp.) Employee group: Full Time Schedule: Monday to Friday 8:30am to 5pm. Weekends may be required. FLSA Status: Non-Exempt Location: School of Medicine Campus Department name: SOM DOM Pulmonary Personnel area: School of Medicine The listed salary range represents the minimum and maximum Johns Hopkins University offers for this position, based on a good faith estimate at the time of posting. Actual compensation will vary depending on factors such as location, skills, experience, market conditions, education, and internal equity. Not all candidates will qualify for the highest salary in the range. Johns Hopkins provides a comprehensive benefits package supporting health, career, and retirement. Learn more: https://hr.jhu.edu/benefits-worklife/. Equal Opportunity Employer All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran. EEO is the Law https://www.eeoc.gov/sites/default/files/2023-06/22-088_EEOC_KnowYourRights6.12ScreenRdr.pdf