About the Work Unit:The Lab in the Department of Biochemistry at Vanderbilt University is seeking a biochemistry research assistant to aid in the lab’s study of biomolecular condensates.  Biomolecular condensates are macroscopic structures that function to spatially organize biological functions and biochemical reactions within the cell. The lab is interested in elucidating mechanisms by which biomolecular condensates are utilized to execute proper animal embryogenesis and development.  To understand the function, structure, and regulation of biomolecular condensates the lab utilizes multidisciplinary approaches including biochemistry, biophysical approach, genome editing, cell biology, single-molecule imaging, and super-resolution imaging.  The work unit is made up of faculty, undergraduate students, and graduate students.Key Functions and Expected Performances:

Assist in Research and Development  

Preparation of buffers and reagents 

Writing and updating laboratory protocols  

Production of DNA vectors for protein expression 

Overexpression and purification of proteins from bacterial and insect cells for biophysical characterization 

Use biophysical methods to characterize interactions of proteins 

Use microscopy approach to characterize cellular phenomena and biochemical reactions

Writing and updating laboratory protocols  

Collecting and assembling research data for presentation and/or reports 

Participating in weekly meetings to discuss project progress 

Lab space maintenance 

Assists with orders and maintenance of inventory and supplies

Other tasks as directed by the supervisor

Supervisory Relationships:

No supervisory responsibilities

The position reports administratively and functionally to the PI.

Education and Certifications:A Bachelor’s degree in cellular biology, molecular biology, biochemistry, or a related field from an accredited institution of higher education is necessary.Experience and Skills:Ability to perform all basic calculations related to buffer composition (Molarity, dilution factor, etc.) is necessary.Ability to be organized, have excellent verbal and written communication skills, and show great attention to detail is necessary.