We are seeking a **_Research Specialist II_** , for the new laboratory in the Division of Pediatric Hematology in the Department of Pediatrics, which is focused on the development and application of cutting-edge nucleic acid delivery and genome engineering tools to mammalian cell and mouse models of disease. The successful candidate will provide administrative and project support to ensure efficient and effective deliverables for the team. The Research Specialist II will assist the Director in the inventory, storage and recovery of biologic samples, ELISAs, cell culture, laboratory maintenance, assisting students and other trainees in laboratory procedures. **Specific duties & responsibilities** _Scientific Responsibilities Experimental_ + Optimize and conduct molecular biology, cell culture, and mouse model experiments. + Develop and design genome editing therapeutics and lipid nanoparticle formulation. + Production of genome editor mRNA and lipid nanoparticle formulation, conduct quality control. + Assess efficacy and safety of the therapeutic. + Conduct statistical analysis and report on outcomes for publication. + Independently optimize, perform, and analyze data from high-level assays including digital PCR, next-generation sequencing, and CUT&RUN. + Use scientific knowledge and lab experience to make judgments and decisions that define research objectives. + Make scientific contribution to protocol development, efficiency, and productivity. + Follow established procedures or protocols and utilizes aseptic technique to perform laboratory tests and/or experiments which include: cell culture, immunohistochemistry, immunocytochemistry, microscopy, and other basic molecular biology techniques. + Use various software applications and graphics packages to assemble, manipulate and/or format data and/or test reports. + Participate in the organization and operation of the laboratory. + Perform general lab maintenance duties, may need to order and manage supplies and reagents of the lab. + Prepares solutions, reagents, and stains following standard laboratory formulas and procedures. _Administrative Responsibilities_ + Contribute to writing peer-reviewed manuscripts and grant proposals. + Communicate with collaborators to plan experiments and discuss results. + Process analyzed data into figures for scientific publications and presentations. + Order oligonucleotide libraries, synthetic genes, and lab supplies. + Record research data activity. **Minimum Qualifications** + Bachelor's Degree in Biology, Chemistry or related field. + Two years laboratory experience performing relevant techniques. + Additional education may substitute for required experience and additional related experience may substitute for required education beyond HS Diploma/Graduation Equivalent, to the extent permitted by the JHU equivalency formula. **Preferred Qualifications** + Experience with molecular biology (including advanced) techniques, cell culture, and mouse work. + Working knowledge of one or more of the following disciplines achieved through undergraduate studies and laboratory experience: biology, biochemistry, chemistry, physics, microbiology and/or genetics. + Able to work in BSL2+ cell culture and mouse facilities. Classified Title: Research Specialist II Role/Level/Range: ACRP/03/MB Starting Salary Range: $41,300 - $72,300 Annually ($54,000 targeted; Commensurate w/exp.) Employee group: Full Time Schedule: M-F; 8:30 - 5pm FLSA Status: Exempt Location: School of Medicine Campus Department name: SOM Ped Hematology Personnel area: School of Medicine The listed salary range represents the minimum and maximum Johns Hopkins University offers for this position, based on a good faith estimate at the time of posting. Actual compensation will vary depending on factors such as location, skills, experience, market conditions, education, and internal equity. Not all candidates will qualify for the highest salary in the range. Johns Hopkins provides a comprehensive benefits package supporting health, career, and retirement. Learn more: https://hr.jhu.edu/benefits-worklife/. Equal Opportunity Employer All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran. EEO is the Law https://www.eeoc.gov/sites/default/files/2023-06/22-088_EEOC_KnowYourRights6.12ScreenRdr.pdf