We are seeking a proficient **_Research Specialist_** with a sophisticated understanding of specific, advanced techniques and a solid foundation in the underlying science, gained from professional experience. Works independently and manages daily research projects. The research specialist participates in planning experiments and evaluating/interpreting test results utilizing detailed knowledge of research laboratory procedures and practices. Capable of independent judgment in the selection, development, performance and evaluation of research methods; operates and maintains appropriate equipment. **Specific Duties and Responsibilities** + Performs cell culture and processes biofluids and tissues from human donors and animal models. Performs fast protein liquid chromatography (FPLC)-based size separation to obtain proteins and protein complexes, confirming identity of selected isolated proteins. Isolates RNA, performs quality control, and prepares RNA sequencing libraries using methods including advanced, ligation-free preparations. Separation and characterization of extracellular vesicles. Assists with analysis and graphical representation of RNA-seq data. Troubleshoots and optimizes techniques based on scientific knowledge and research experience. Liaises with bioinformatics collaborators. + Follows established procedures or protocols and utilizes aseptic and RNase-free technique to perform laboratory tests and/or experiments which include conventional and real-time PCR, electrophoretic separations (agarose and acrylamide gels), Western blot, ELISA, cloning and/or subcloning, extracellular vesicle isolation and characterization, electron microscopy, nanoparticle tracking analysis, resistive pulse sensing, flow cytometry, and other molecular and cellular biology techniques. + Learn and train in additional procedures in order to expand skills, foster individual growth, and directly contribute to and facilitate laboratory success. + Uses various software applications, such as spreadsheets, relational databases, electronic mail, World Wide Web, statistical packages, and graphics packages to assemble, manipulate and/or format data and/or test reports. + Participates in the organization and operation of the laboratory, including oversight of undergraduate student researchers. Performs general lab maintenance duties and maintains excellent written records and electronic databases. + Uses universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens. Completes annual university biohazard/universal precaution/radiation safety training, as appropriate. Monitors biohazard/radiation safety standards and proper handling of potentially hazardous chemical and biological agents and/or radiation sources in the workplace. **Special Knowledge, Skills, and Abilities** + Candidates should be motivated, highly organized, have good communication and time management skills, and work effectively in a team setting. + A willingness to learn new skills is appreciated. **Minimum Qualifications** + Bachelor's Degree in Biology, Chemistry or related field. + One year laboratory experience performing relevant techniques. + Master's Degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula. **Preferred Qualifications** + One year of laboratory experience performing relevant techniques. + Master’s Degree, with related graduate research, may substitute for experience. Classified Title: Research Specialist Role/Level/Range: ACRP/03/MA Starting Salary Range: $17.11 - $29.90 HRLY ($62,200 targeted; Commensurate w/exp.) Employee group: Full Time Schedule: Monday - Friday, 40 hours per week FLSA Status: Non-Exempt Location: School of Medicine Campus Department name: SOM Comp Med Retrovirus Bio Laboratory Personnel area: School of Medicine The listed salary range represents the minimum and maximum Johns Hopkins University offers for this position, based on a good faith estimate at the time of posting. Actual compensation will vary depending on factors such as location, skills, experience, market conditions, education, and internal equity. Not all candidates will qualify for the highest salary in the range. Johns Hopkins provides a comprehensive benefits package supporting health, career, and retirement. Learn more: https://hr.jhu.edu/benefits-worklife/. Equal Opportunity Employer All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran. EEO is the Law https://www.eeoc.gov/sites/default/files/2023-06/22-088_EEOC_KnowYourRights6.12ScreenRdr.pdf