The Meydan Lab is interested in the mechanisms of protein synthesis, and the role of translation regulation and ribosomes in human health. We use various biochemistry techniques, sequencing of ribosome protected mRNA fragments, (a.k.a ribosome profiling (Ribo-seq), disome profiling (Disome-seq), and mitoribosome profiling), and RNA-seq in model systems of Saccharomyces cerevisiae (budding yeast), immortalized human cells, and neurons differentiated from induced pluripotent stem cells (iPSCs) to address our questions.
Key Functions and Expected Performance:
Assists in Research and Development:
Collects data using appropriate lab experiments, procedures, and techniques
Maintains knowledge of current literature on Meydan Lab’s research focus
Prepares buffers and reagents
Generates and maintains plasmids, yeast strains and mammalian cell lines
Analyzes and interprets data with assistance of other members of the laboratory
Writes and updates laboratory protocols
Reports on the status of research activities in oral and written formats
Assembles and compiles research data for presentations and/or publications
Maintenance
Conducts general lab maintenance
Assists in set up, operation, and maintenance of laboratory equipment including, but not limited to – biosafety cabinets, incubators, centrifuges, etc.
Performs administrative duties
Monitors inventory of supplies and assists with ordering
Participates in one-on-one meetings and group lab meetings to discuss project progress
Other tasks as directed by supervisor
Supervisory Relationships:
This position does not have supervisory responsibility.
The position reports administratively and functionally to the PI.
Education and Certifications:
A Bachelor’s degree in biochemistry, biological sciences, chemistry, or related field from an accredited institution of higher education is necessary.
Experience and Skills:
Knowledge and experience with mammalian cell culture, iPSC and neurons is preferred.
Knowledge and experience with plasmid cloning is preferred.
Knowledge and experience with next generation sequencing is preferred.
Knowledge and experience of yeast growth and maintenance techniques is preferred.
Experience with organization, inventory, and record keeping is preferred.
Excellent organizational and communications skills (both verbal and written) are preferred.