Our client, one of the largest pharmaceutical companies in USA is looking for an Engineer - II with expertise in Protein (Purification, characterization)/DNA to work at Elkton, VA. **Job Title:** Engineer II (Protein/DNA) **Duration:** 12 Month **Location:** Onsite - 2778 South East Side Highway, Elkton, VA 22827 **Pay rate:** $40 - $44 per hour **Ideal candidate would be a fresh PhD or masters candidate with industry or academic lab experience in protein purification, AKTA chromatography, TFF filtration etc. Key aspect is experience. Designing, troubleshooting, running experiments.** **Qualifications:** **Education Minimum Requirement:** + A bachelor’s (w/4+ yrs. purification experience) or masters (2+ yrs purification experience) or Ph.D. degree (direct experience) in a relevant scientific discipline (biochemistry, chemistry, biology), life sciences, Engineering **Required Experience and Skills** + **Experience with protein purification from bacterial or yeast or mammalian systems in a laboratory or pilot or commercial scale.** + **Hands on experience using AKTA Purification Systems** + **Strong background in protein biochemistry** + **Experience with biochemical and analytical methods for protein and or DNA analysis.** + Wet chemistry laboratory skills (pipetting, buffer preparation, filtration, dilution, stoichiometry) + Proficiency with technical writing and laboratory records (laboratory notebooks, chemical inventory, data management) + Excellent collaborative and team-working skills + Strong technical problem-solving abilities, data interpretation and presentation competence **Preferred Experience and Skills:** + Experience working with protein purification + Experience working with column chromatography and AKTA/Unicorn systems + Experience working with tangential flow filtration processing – microfiltration/ultrafiltration + Experience performing fermentation or cell culture studies at the labs scale + Experience performing fermentation or cell culture studies using shake flasks + Knowledge of yeast fermentation and metabolism + Experience working in GMP and GLP environments + Experience with laboratory operations + Experience with facility operations + Experience working in an industry environments + Experience conducting laboratory scale process characterization studies + Experience troubleshooting technical issues (experimental, equipment-related issues) + Experience with material characterization + Experience working with enzymes and peptides + Experience in method development and method transfer + Experience with six sigma, 5S principles **Responsibilities:** + This position will report to the Technical Operations Department and will support the manufacture of HPV Gardasil as part of a multidisciplinary team, the candidate will have a fundamental knowledge of fermentation and cell culture, protein chemistry and purification, analytical techniques and/or laboratory operations. + Join our client's Process Development Team in using a variety of fermentation techniques, purification techniques, and characterization methods to support commercial and bench scale pharmaceutical processes. + The PD team uses a scale down model to provide investigation support, promote process understanding, and facilitate technology transfer of products to the Elkton site. + Work with teams across the network to bridge process, quality, and analytical principles and synthesize these into a focused laboratory that provides high quality support to realize the sustained production of medication for patients. **As a member of Process Development, the primary goal of the position is to:** + Characterize proteins, VLPs and constituent components with a variety of analytical techniques + Participate in/drive fermentation and/or purification of proteins/VLPs at lab and pilot scale + Provide support for execution of process development activities and manufacturing investigations + Perform laboratory scale studies + Design and troubleshoot experiments + Employ excellent verbal and written communication skills • Author laboratory notebooks and technical documents + Work both independently and within multidisciplinary team + Produce high quality work against firm deadlines + Maintain good sample handling practices + Balance and prioritize competing deliverables + Commit to creating a diverse and inclusive workplace If this is a role that interests you and you’d like to learn more, click apply now and a recruiter will be in touch with you to discuss this great opportunity. 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